English Version

Characterization of novel microsatellite markers of the Emei Shan Liocichla using restriction site-associated DNA sequencing

2017-12-24 20:00:22

论文题目:
Characterization of novel microsatellite markers of the Emei Shan Liocichla using restriction site-associated DNA sequencing
第一作者:
Ailin Yang
文章摘要:

Background The Emei Shan Liocichla (Liocichla omeiensis) is an endemic bird species to southwestern China with a small geographic range. However, little was known about the genetic status of this threatened species. Methods We applied restriction-site-associated DNA sequencing (RAD-Seq) for rapid mass identification of microsatellite markers of the Emei Shan Liocichla. ResultsA total of 11,564 microsatellite sequences were obtained, 600 random loci were designed for screening and 24 polymorphic microsatellite loci were selected for further validation. The average allele number, average observed heterozygosity and average expected heterozygosity were relatively low in our samples, which were 6.08, 0.6618 and 0.7048, respectively, indicating that the Emei Shan Liocichla might have lost some genetic diversity. Further analyses suggested that the populations distributed on two mountains (Daxiangling and Xiaoliangshan) showed a modest degree of genetic differentiation. Conclusions These novel microsatellite markers provided valuable preliminary knowledge regarding the genetic status of the Emei Shan Liocichla and can be useful in further studies, as well as in the management and conservation of this species. 


发表年度:
2017
刊物名称:
Avian Research
通讯作者:
Zheng-Wang Zhang

北京师范大学版权所有 联系地址:北京市海淀区新街口外大街19号 北京师范大学生物多样性与生态工程教育部重点实验室 邮编100875