English Version

Effects of acute cold exposure on oxidative balance and total antioxidant capacity in juvenile Chinese soft-shelled turtle, Pelodiscus sinensis

2017-12-24 19:23:48

论文题目:
Effects of acute cold exposure on oxidative balance and total antioxidant capacity in juvenile Chinese soft-shelled turtle, Pelodiscus sinensis
第一作者:
Wenyi Zhang
文章摘要:

Acute cold exposure may disturb physiological homeostasis of the body in ectotherms. By now, there is no information about the effects of cold exposure on homeostasis of reactive oxygen species (ROS) or antioxidant defense response in the Chinese soft-shelled turtle, Pelodiscus sinensis. In this study, P.sinensis juveniles were acclimated at 28 °C, transferred to 8 °C as cold exposure for 12 hours, moved back to 28 °C rewarming for 24 hours. We measured ROS level and total antioxidant capacity (TAC) in the brain, liver, kidney and spleen at 2 hours, 12 hours cold exposure, and the end of rewarming. Malonaldehyde (MDA) and carbonyl protein were used as marker of oxidative damage. Turtles being maintained simultaneously at 28°C all the time were used as the control group. Cold exposure did not disturb ROS balance in all four tissues, while rewarming raised ROS level in the brain and kidney of P. sinensis. Cold exposure and rewarming decreased TAC in the brain, liver and spleen while didn't change TAC in the kidney. MDA and carbonyl protein levels did not increase during the treatment, indicating no oxidative damage in all four tissues of P. sinensis. Our results indicated that a harsh cold exposure did not break the inner oxidative balance of P. sinensis, but more ROS produced during rewarming. P. sinensis showed good tolerance to the harsh temperature change by effective protection of its antioxidant defense system to oxidative damage. This study provides basic data to stress biology of P. sinensis. This article is protected by copyright. All rights reserved


发表年度:
2017
刊物名称:
Integrative Zoology
通讯作者:
Wenyi Zhang

北京师范大学版权所有 联系地址:北京市海淀区新街口外大街19号 北京师范大学生物多样性与生态工程教育部重点实验室 邮编100875